Jump to content

Station Science 101: Studying DNA in Space


NASA

Recommended Posts

  • Publishers

Long-term space exploration exposes humans to radiation that can damage deoxyribonucleic acid or DNA, which carries the genetic information for our development and functioning. Conditions in space also affect the way the body repairs such damage, potentially compounding the risk. Research on the International Space Station studies DNA damage and repair using tools and techniques to sequence, analyze, and even edit DNA.

Those tools and techniques have been developed especially for use in space, which has unique safety considerations and where there are limits on the size and weight of equipment. This specialization has made this type of research possible and resulted in significant milestones in DNA research.

Rubins wears a black sweatshirt as she holds a small experiment tube and smiles at the camera. There are two laptops in front of her and equipment and wiring above her.
NASA astronaut Kate Rubins prepares a run of Biomolecule Sequencer experiment, which sequenced DNA in space for the first time.
NASA

In April 2016, ESA (European Space Agency) astronaut Tim Peake first amplified DNA using the first polymerase chain reaction (PCR) device sent to station, called miniPCR.1 An important step in the process of analyzing genetic material, amplification involves making multiple copies of a segment of DNA. NASA astronaut Kate Rubin sequenced DNA in space for the first time in August 2016 using a commercial off-the-shelf device called MinION.2 In August 2017, NASA astronaut Peggy Whitson combined the miniPCR and MinION to identify the first unknown microbe from the station, validating a process that could make possible in-flight identification of microbes and diagnosis of infectious diseases on future missions.3 In August 2018, NASA astronaut Ricky Arnold first used a “swab to sequencer” DNA sequencing method that eliminates the need to culture bacteria before analysis.4

Arnold, facing the camera, wears a blue shirt, glasses, and light blue gloves. His right hand holds the miniPCR on the work bench. A laptop and video camera are visible behind him.
NASA astronaut Ricky Arnold processes DNA from swabs of space station surfaces to identify microbes.
NASA

Another milestone, reached in May 2019, was the first CRISPR gene editing on station, performed by NASA astronaut Christina Koch.5 CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. These are short, repeated sequences of DNA noted in bacteria with viral DNA sequences in between them. Bacteria transcribe the viral DNA sequences to RNA, which then guides a specific protein to the viral DNA and cuts it – creating a line of defense against invading viruses. Researchers can create a guide RNA to be specific to any part of a genome. This means CRISPR can be used to create precise breaks in a known location of a gene, resulting in simplified gene editing.

A program called Genes in Space has employed these advances for multiple investigations. A collaboration between Boeing and miniPCR bio sponsored by the ISS National Lab and New England Biolabs, this program is a national contest where students in grades 7 through 12 design DNA analysis experiments for the space station.

Genes in Space-6 used CRISPR to successfully generate breaks in the DNA of a common yeast, allow for repair of the breaks, and sequence the patched-up DNA to determine whether its original order was restored, all during spaceflight.5 Performing the entire process in space – rather than causing a break, freezing the sample, and sending it into space to repair –provided researchers insight into the type of repair mechanism used. Organisms repair DNA breaks in one of two major ways. One method may add or delete bases while the other rejoins the strands without changing the DNA sequence. Understanding whether one type of repair is less error-prone has important implications for protecting crew members.

Koch faces a work bench and concentrates on sample tubes in her left hand. She is wearing a headset, gloves, and glasses.
NASA astronaut Christina Koch works on the Genes in Space-6 experiment.
NASA

Genes in Space-5 represented an important step toward a rapid, safe, and cost-effective way to examine the immune system during spaceflight. This investigation also provided proof of concept for simultaneously amplifying multiple DNA sequences in space, expanding the possibilities for in-flight research and health monitoring.

Genes in Space-10 validated a method for measuring and analyzing the length of DNA fragments known as telomeres using fluorescence. Telomeres, cap-like genetic structures at the end of chromosomes that protect them from damage, shorten with age but have been found to lengthen in space. Analyzing telomere length could help determine the mechanism behind this effect. Results from the investigation also could provide a way to measure DNA and to diagnose genetic-based medical problems during spaceflight. Sending DNA samples back to Earth for analysis can cause the samples to degrade and is not feasible for future long-duration missions. Insight into why telomeres lengthen in space could lead to a better understanding of their role in human aging as well.

Having an entire molecular laboratory in space greatly increases what scientists can do. The ability to analyze DNA, study how it is damaged and repaired in space, and make specific changes to it enables more complex research. Identifying unknown organisms and changes in known ones is key to keeping crew members safe on future missions.

Melissa Gaskill
International Space Station Program Science Office
Johnson Space Center

Search this database of scientific experiments to learn more about those mentioned above.

Citations

1 Boguraev, A. S. et al. Successful amplification of DNA aboard the International Space Station. NPJ Microgravity 3, 26, doi:10.1038/s41526-017-0033-9 (2017).

2 Castro-Wallace, S. L. et al. Nanopore DNA Sequencing and Genome Assembly on the International Space Station. Sci Rep 7, 18022, doi:10.1038/s41598-017-18364-0 (2017).

3 Burton, A. S. et al. Off Earth Identification of Bacterial Populations Using 16S rDNA Nanopore Sequencing. Genes (Basel) 11, doi:10.3390/genes11010076 (2020).

4 Stahl-Rommel, S. et al. Real-Time Culture-Independent Microbial Profiling Onboard the International Space Station Using Nanopore Sequencing. Genes (Basel) 12, doi:10.3390/genes12010106 (2021).

5 Stahl-Rommel, S. et al. A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station. PloS one 16, e0253403, doi:10.1371/journal.pone.0253403 (2021).

View the full article

Link to comment
Share on other sites

Join the conversation

You can post now and register later. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.

Guest
Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.

  • Similar Topics

    • By European Space Agency
      Image: The Copernicus Sentinel-2 mission takes us over Riyadh, the capital city of Saudi Arabia. View the full article
    • By NASA
      3 min read
      NASA Selects Two Teams to Advance Life Sciences Research in Space 
      NASA announced two awards Thursday to establish scientific consortia – multi-institutional coalitions to conduct ground-based studies that help address the agency’s goals of maintaining a sustained human presence in space. These consortia will focus on biological systems research in the areas of animal and human models, plants, and microbiology. When fully implemented, the awards for these consortia will total about $5 million. 
      Space biology efforts at NASA use the unique environment of space to conduct experiments impossible to do on Earth. Such research not only supports the health and welfare of astronauts, but results in breakthroughs on diseases such as cancer and neurodegenerative disorders to help protect humanity down on the ground.  
      The awards for the two consortia are for the following areas:  
      Studying space biosphere. The Biology in Space: Establishing Networks for DUrable & REsilient Systems consortium involves a collaborative effort between human/animal, plant, and microbial biologists to ensure an integrated view of the space flight biosphere by enhancing data acquisition, modeling, and testing. It will include participation of more than thirty scientists and professionals working together from at least three institutions. Led by Kristi Morgansen at the University of Washington in Seattle, Washington.    Converting human waste into materials for in-space biomanufacturing. The Integrative Anaerobic Digestion and Phototrophic Biosystem for Sustainable Space Habitats and Life Supports consortium will develop an anaerobic digestion process that converts human waste into organic acids and materials that can be used for downstream biomanufacturing applications in space. It will include eight scientists from six different institutions in three different states, including Delaware and Florida. The consortium is led by Yinjie Tang at Washington University in St. Louis, Missouri.    Proposals for these consortia were submitted in response to ROSES 2024 Program Element E.11 Consortium in Biological Sciences for a consortium with biological sciences expertise to carry out research investigations and conduct activities that address NASA’s established interests in space life sciences.  
      NASA’s Space Biology Program within the agency’s Biological and Physical Sciences division conducts research across a wide spectrum of biological organization and model systems to probe underlying mechanisms by which organisms acclimate to stressors encountered during space exploration (including microgravity, ionizing radiation, and elevated concentrations of carbon dioxide). This research informs how biological systems regulate and sustain growth, metabolism, reproduction, and development in space and how they repair damage and protect themselves from infection and disease. 
      For more information about NASA’s fundamental space-based research, visit https://science.nasa.gov/biological-physical
      Share








      Details
      Last Updated Oct 17, 2024 Contact NASA Science Editorial Team Location NASA Headquarters Related Terms
      Biological & Physical Sciences For Researchers Research Opportunities in Space and Earth Sciences (ROSES) Science & Research View the full article
    • By NASA
      4 min read
      Preparations for Next Moonwalk Simulations Underway (and Underwater)
      Jacquelyn Shuman visually assesses a prescribed fire at Ft. Stewart in Georgia, working with partner organizations as part of the Department of Defense Ft. Stewart 2024 Fire Research Campaign. USFS/Linda Chappell Jacquelyn Shuman, FireSense Project Scientist at NASA Ames Research Center, originally wanted to be a veterinarian. By the time she got to college, Shuman had switched interests to biology, which became a job teaching middle and high school science. Teaching pivoted to finance for a year, before Shuman returned to the science world to pursue a PhD.

      It was in a forest ecology class taught by her future PhD advisor, Herman “Hank” Shugart, that she first discovered a passion for ecosystems and dynamic vegetation that led her into the world of fire science, and eventually to NASA Ames.

      While Shuman’s path into the world of fire science was not a direct one, she views her diverse experiences as the key to finding a fulfilling career. “Do a lot of different things and try a lot of different things, and if one thing isn’t connecting with you, then do something different,” Shuman said.

      Diving into the World of Fire

      Shuman’s PhD program focused on boreal forest dynamics across Russia, examining how the forest changes in response to climate change and wildfire. During her research, she worked mainly with scientists from Russia, Canada, and the US through the Northern Eurasia Earth Science Partnership Initiative (NEESPI), where Shugart served as the NEESPI Chief Scientist. “The experience of having a highly supportive mentor, being a part of the NEESPI community, and working alongside other inspiring female scientists from across the globe helped me to stay motivated within my own research,” Shuman said.

      After completing her PhD, Shuman wanted to become involved in collaborative science with a global impact, which led her to the National Center for Atmospheric Research (NCAR). There, she spent seven years working as a project scientist on the Next Generation Ecosystem Experiment NGEE-Tropics) on a dynamic vegetation model project called FATES (Functionally Assembled Terrestrial Ecosystem Simulator). As part of the FATES team, Shuman used computer modeling to test vegetation structure and function in tropical and boreal forests after wildfires, and was the lead developer for updating the fire portion of the model.

      This figure shows fire characteristics from an Earth system model that uses vegetation structure and interactive fire. The FATES model captures the fire intensity associated with burned land and grass growth in the Southern Hemisphere. Shuman et al. 2024 GMD Fire has also played a powerful role in Shuman’s personal life. In 2021, the Marshall Fire destroyed neighborhoods near her hometown of Boulder, Colorado, causing over $513 million of damage and securing its place as the state’s most destructive wildfire. Despite this, Shuman is determined to not live in fear. “Fire is part of our lives, it’s a part of the Earth system, and it’s something we can plan for. We can live more sustainably with fires.” The way to live safely in a fire-inclusive ecosystem, according to Shuman, is to develop ways to accurately track and forecast wildfires and smoke, and to respond to them efficiently: efforts the fire community is continuously working on improving.

      The Fire Science Community

      Collaboration is a critical element of wildland fire management. Fire science is a field that involves practitioners such as firefighters and land managers, but also researchers such as modelers and forecasters; the most effective efforts, according to Shuman, come when this community works together. “People in fire science might be out in the field and carrying a drip torch and marching along in the hilltops and the grasslands or be behind a computer and analyzing remote sensing data,” Shuman said. “We need both pieces.”

      Protecting communities from wildfire impacts is one of the most fulfilling aspects of Shuman’s career, and a goal that unites this community. “Fire research poses tough questions, but the people who are thinking about this are the people who are acting on it,” Shuman said. “They are saying, ‘What can we do? How can we think about this? What information do we need? What are the questions?’ It’s a special community to be a part of.”

      Looking to the Future of Fire

      Currently at NASA Ames Research Center, Shuman is the Project Scientist for FireSense: a project focused on delivering NASA science and technology to practitioners and operational agencies. Shuman acts as the lead for the project office, identifying and implementing tools and strategies. Shuman still does ecosystem modeling work, including implementing vegetation models that forecast the impact of fire, but also spends time traveling to active fires across the country so she can help partners implement NASA tools and strategies in real time.

      FireSense Project Scientist Jacquelyn Shuman stands with Roger Ottmar (United States Forest Service), surveying potential future locations for prescribed burns in Fishlake National Forest. NASA Ames/Milan Loiacono
      “Right now, many different communities are all recognizing that we can partner to identify the best path forward,” Shuman said. “We have an opportunity to use everyone’s strengths and unique perspectives. It can be a devastating thing for a community and an ecosystem when a fire happens. Everyone is interested in using all this collective knowledge to do more, together.”


      Written by Molly Medin, NASA Ames Research Center

      Share
      Details
      Last Updated Oct 17, 2024 Related Terms
      General Earth Science Earth Science Division Explore More
      4 min read Navigating Space and Sound: Jesse Bazley Supports Station Integration and Colleagues With Disabilities
      Article 18 hours ago 3 min read Sacrifice and Success: NASA Engineer Honors Family Roots
      Article 19 hours ago 7 min read What is a Coral Reef?
      Article 2 days ago Keep Exploring Discover Related Topics
      Missions
      Humans in Space
      Climate Change
      Solar System
      View the full article
    • By NASA
      NASA astronaut and Expedition 72 Flight Engineer Nick Hague in the space station cupola. (Credit: NASA) Students from Iowa will have the opportunity to hear NASA astronaut Nick Hague answer their prerecorded questions while he’s serving an expedition aboard the International Space Station on Monday, Oct. 21.
      Watch the 20-minute space-to-Earth call at 11:40 a.m. EDT on NASA+. Students from Iowa State University in Ames, First Robotics Clubs, World Food Prize Global Youth Institute, and Plant the Moon teams will focus on food production in space. Learn how to watch NASA content on various platforms, including social media.
      Media interested in covering the event must contact Angie Hunt by 5 p.m., Friday, Oct.18 at amhunt@iastate.edu or 515-294-8986.
      For more than 23 years, astronauts have continuously lived and worked aboard the space station, testing technologies, performing science, and developing skills needed to explore farther from Earth. Astronauts aboard the orbiting laboratory communicate with NASA’s Mission Control Center in Houston 24 hours a day through SCaN’s (Space Communications and Navigation) Near Space Network.
      Important research and technology investigations taking place aboard the space station benefit people on Earth and lays the groundwork for other agency missions. As part of NASA’s Artemis campaign, the agency will send astronauts to the Moon to prepare for future human exploration of Mars; inspiring Artemis Generation explorers and ensuring the United States continues to lead in space exploration and discovery.
      See videos and lesson plans highlighting space station research at:
      https://www.nasa.gov/stemonstation
      -end-
      Abbey Donaldson
      Headquarters, Washington
      202-358-1600
      Abbey.a.donaldson@nasa.gov
      Sandra Jones 
      Johnson Space Center, Houston
      281-483-5111
      sandra.p.jones@nasa.gov
      View the full article
    • By NASA
      Science Launching on SpaceX's 31st Cargo Resupply Mission to the Space Station
  • Check out these Videos

×
×
  • Create New...